Contact: Paul Stemmer, CURES ESFC Co-Lead and Proteomics Core Director ; Joe Caruso, CURES member and Proteomics Core Associate Director
Proteomic analyses are available through the Proteomics Core at WSU. A full range of mass spectrometry-based standard proteomic services are supported. In addition, the ESFC uses advanced proteomics technologies to identify and quantify protein adducts that occur as a consequence of environmental toxicant exposure. CURES researchers have detected adducts on serum albumin and hemoglobin; the two most abundant proteins in blood that act as buffers for reactive chemical intermediates. Analysis of adducts on albumin and hemoglobin can provide information on environmental exposures occurring as long as 60 to 90 days prior to sample collection.
The Proteomics Core Facility provides state-of-the-art technology for characterization of protein identity, modification and differential expression.
The Proteomics Core Facility provides the following services and technologies:
- Protein identification using orbitrap MS with nanoflow ultra high performance liquid chromatography.
- Protein quantitation using spectral counting, isobaric tags, stable isotope labeling with amino acids in cell culture (SILAC) with data acquired on the Orbi Fusion or Q Exactive, or the multiple reaction monitoring (MRM) strategy using the TSQ Vantage system
- Proteomic profiling using two-dimensional chromatographic separations or MuDPIT technologies
- Analysis of post-translational modifications using nano-LC-MS/MS with fragmentation by electron transfer dissociation (ETD), higher energy collisional dissociation (HCD), or collision-induced dissociation (CID).
- Analysis of adducts on proteins and peptides using affinity selection and/or affinity depletion followed by LC-MS/MS.
- Robotic protein digestion using the Genomic Solutions Investigator ProGest
- Data analysis using Mascot, Sequest, X!tandem, MaxQuant, and Peaks algorithms with data compilation and secondary analysis using Scaffold and R scripts.
