Genomics and Epigenomics:
CURES Contact:
Doug Ruden, ESFC Co-Lead and Director of Epigenomics
A full range of genome, transcriptome and epigenome analysis services are available to CURES Investigators through the Applied Genomics Technology Center at WSU. A Fluidigm C1 instrument for single cell RNA sequencing (RNA-seq) has been purchased and is being used for CURES projects. The purchase was in response to a CURES facility core usage survey that polled Center members' current and anticipated research needs. The Fluidigm C1 takes exposure science technology to the single cell level. It uses an alternative to traditional flow cytometry, utilizing microfluidics to separate single cells from test specimens in order to prepare libraries for RNA-seq or other types of genomic analyses. For example, using the C1 System, mixed populations of neurons and astrocytes can be analyzed at the single cell level with high resolution. This enables the detection of dramatic variations in gene expression that occur as a consequence of toxicant exposure.
Genomic and Epigenomic analyses are handled through the Applied Genomics Technology Center. The AGTC is a state-of-the-art, fee-for-service genomics center that provides a wide range of genomic technologies to the medical research community.
The AGTC offers the following services:
- RNA Isolation
- Analysis if DNA and RNA Expression
- DNA Isolation
- DNA Methylation Studies
- Arrayed Comparative Genomic Hybridization Studies
- Somatic Mutation Analysis
- Next-generation Sequencing
- DNA sequencing(Sanger)
- Genotyping Services
- Genomics Quality Control and Reporting
- Single Cell RNA-seq
- Single Cell ATAC-seq
Bioinformatics
Within CURES, Bioinformatics is offered by the AGTC for genomic and epigenomic data and the Proteomics Core for Proteome data sets.
CURES Contacts:
Doug Ruden, CURES ESFC Co-Lead and Director of Epigenomics
Alan Dombowski, CURES Member: Interpretation of proteomic data sets
Samiran Ghosh, CURES IHSFC Co-Lead: Statistical evaluation of proteomic data sets
Nicholas Carruthers, CURES Member: Implementing software programs used in proteomic analysis
Interested in these services?
